Journal: Cells

Article Title: Effect of Long-Term 3D Spheroid Culture on WJ-MSC

doi: 10.3390/cells10040719

Figure Lengend Snippet: List of primary antibodies used for immunocytochemistry.

Article Snippet: Nestin , Mouse monoclonal , IgG1 , 1:500 , Merck Millipore , MAB5326.

Techniques: Immunocytochemistry

Journal: PLoS ONE

Article Title: Development of novel monoclonal antibodies with specific binding affinity for denatured human CD26 in formalin-fixed paraffin-embedded and decalcified specimens

doi: 10.1371/journal.pone.0218330

Figure Lengend Snippet: MSTO parent, MSTO-CD26, JMN CD26-shRNA, JMN ctrl-shRNA, A549, Jurkat parent or Jurkat-CD26 cells were incubated with unlabeled isotype control or purified mouse anti-human CD26 mAb (19–32, U16-3 or U38-8) or purified goat anti-human CD26 pAb (R&D Systems), and subsequently stained with PE-labeled goat anti-mouse Ig pAb or PE-labeled donkey anti-goat IgG Ab, and analyzed by flow cytometry. PE-labeled commercial mouse anti-human CD26 mAb (BD Biosciences, clone M-A261) was utilized as a positive control. Representative data of three independent experiments are shown as mean ± S.D. of the mean fluorescence intensity (MFI) from triplicate samples, and similar results were obtained in each experiment.

Article Snippet: In brief, The cultured cell lines were incubated with 10 μg/ml of purified mouse anti-human CD26 mAb (19–32, U16-3 or U38-8) or goat anti-human CD26 pAb or isotype controls for 25 min at 4°C, and subsequently stained with PE-conjugated goat anti-mouse Ig pAb (BD Biosciences, San Jose, CA) or PE-conjugated donkey anti-goat IgG Ab (Santa Cruz Biotechnology) for 25 min at 4°C.

Techniques: shRNA, Incubation, Purification, Staining, Labeling, Flow Cytometry, Positive Control, Fluorescence

Journal: PLoS ONE

Article Title: Conserved Surface Accessible Nucleoside ABC Transporter Component SP0845 Is Essential for Pneumococcal Virulence and Confers Protection In Vivo

doi: 10.1371/journal.pone.0118154

Figure Lengend Snippet: SP0845 23–350 was expressed in E . coli and purified using Ni-NTA affinity and DEAE sepharose anion-exchange chromatography. The purified recombinant protein was resolved on 12% SDS-PAG and stained with Coomassie brilliant blue R250 (A) or transferred to nitrocellulose membrane and visualized using anti-histidine affinity tag antibody followed by horseradish peroxidase conjugated goat anti-mouse Ig as secondary antibody and 3, 3' diaminobenzidine/ H 2 O 2 as substrate (B). The molecular mass marker (in kDa) is shown on the left of the panels.

Article Snippet: The membrane was blocked with 2% skimmed milk followed by 1 h incubations with primary antibody [polyclonal sera raised in mouse against whole heat-killed TIGR4 (diluted 1 in 1000) or anti-histidine affinity tag (diluted 1 in 1000) antibody (Sigma; Catalogue number: H1029) or mouse anti-SP0845 antibody (diluted 1 in 500)] and horse radish peroxidase conjugated goat anti-mouse Ig antisera (diluted 1 in 1000; BD Biosciences, USA; Catalogue number: 554002) as the secondary antibody at room temperature.

Techniques: Purification, Chromatography, Recombinant, Staining, Marker

Journal: PLoS ONE

Article Title: Conserved Surface Accessible Nucleoside ABC Transporter Component SP0845 Is Essential for Pneumococcal Virulence and Confers Protection In Vivo

doi: 10.1371/journal.pone.0118154

Figure Lengend Snippet: (A) Lysates were prepared (starting with mid-logarithmic phase cultures with similar optical density) from the indicated pneumococcal strains and resolved on a 12% SDS-PAG followed by transfer to nitrocellulose membrane. The blot was probed with mouse anti-SP0845 23–350 polyclonal sera followed by horseradish peroxidase conjugated goat anti-mouse Ig antisera as the secondary antibody. 3, 3' Diaminobenzidiene along with H 2 O 2 was used as the substrate. (B) Immunoblot analysis done using lysates prepared from pneumococci isolated from blood and spleen 18 h after infecting BALB/c mice with 10 6 cfu of ATCC 6303. The blot was probed with mouse anti-SP0845 23–350 sera and developed as described for panel A. The molecular mass marker (in kDa) is shown.

Article Snippet: The membrane was blocked with 2% skimmed milk followed by 1 h incubations with primary antibody [polyclonal sera raised in mouse against whole heat-killed TIGR4 (diluted 1 in 1000) or anti-histidine affinity tag (diluted 1 in 1000) antibody (Sigma; Catalogue number: H1029) or mouse anti-SP0845 antibody (diluted 1 in 500)] and horse radish peroxidase conjugated goat anti-mouse Ig antisera (diluted 1 in 1000; BD Biosciences, USA; Catalogue number: 554002) as the secondary antibody at room temperature.

Techniques: Western Blot, Isolation, Marker